ammonium sulfate fractionation meaning in Chinese
硫酸铵分级分离
Examples
- 30 % ~ 60 % ammonium sulfate fractionation of the crude extract was carried out at 4 ? . the optimum temperature and ph for it were 37 ? and 6 . 5
结果发现，该酶作用的最适ph为6 . 5 ，最适温度为37 ；在ph5 8之间稳定，其热稳定性不高。 - The purified badh , which became a single band in sds - page , has been obtained by a combination of ammonium sulfate fractionation , ion exchange chromatography and gel filtration chromatography
辽宁碱蓬甜菜碱醛脱氢酶分子量为129kd ，单个亚基的分子量为64 . 5kd ，表明其为同型二聚体。 - The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100 . the sod activity for purified enzyme could reach 4966 iu / mg proteins , and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis . when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0 . 2mmol / l kcn or 0 . 5 mmol / l h2o2 , the active band was still showed at the same position , which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通过sds一聚丙烯酸胺凝胶电泳可知该sod酶的分子量约为20kda .在非变性聚丙烯酞胺凝胶( pagb )电泳后，在凝胶son显色反应液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝胶sod显色反应后在原来的sod酶部位仍然含有sod活性带，这表明利用kcn和h20 :处理并不能抑制500的活性，该sod属于mn一sod 。